VAMP8 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51432m-100uL |
| Product Name | VAMP8 Monoclonal Antibody |
| Applications |
WB
ELISA
IHC-F
IF
IHC-P
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This VAMP8 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 2~24 amino acids from the N-terminal region of human VAMP8. |
| Clonality | Monoclonal |
| Clone # | 14C3 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 8673 |
| Swiss Prot | Q9BV40 |
| Synonyms | EDB; VAMP-8; Vesicle-associated membrane protein 8; Endobrevin; VAMP8 |
| Background | SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is VAMP8 Monoclonal Antibody (bsm-51432M) validated for, and at what dilution?
bsm-51432M is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF (1:50-200); IHC-P (1:200-400); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VAMP8 Monoclonal Antibody (bsm-51432M) react with?
Tested reactivity: Human.
How should bsm-51432M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VAMP8 Monoclonal Antibody (bsm-51432M)?
This VAMP8 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 2~24 amino acids from the N-terminal region of human VAMP8.