SUMO3 Monoclonal Antibody

$Lane 1: HL-60 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: 293T\/17 cell lysates probed with SUMO3 Monoclonal Antibody, Unconjugated (bsm-51458M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: HL-60 cell lysates; Lane 2: Jurkat cell lysates; Lane 3: 293T\/17 cell lysates probed with SUMO3 Monoclonal Antibody, Unconjugated (bsm-51458M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51458m
Product Name	SUMO3 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant GST fusion protein encoding human SUMO3.
Clonality	Monoclonal
Clone #	S3G2
Isotype	IgG1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6612
Swiss Prot	P55854
Subcellular location	Cytoplasm, Nucleus
Synonyms	Small ubiquitin-related modifier 3; SUMO-3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B; Smt3B; SUMO3_HUMAN.
Background	Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
Application Dilution
WB	=1:500-2000