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SNAIL Monoclonal Antibody

Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: NCI-H460 cell lysates probed with SNAIL Monoclonal Antibody, Unconjugated (bsm-51463M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: NCI-H460 cell lysates probed with SNAIL Monoclonal Antibody, Unconjugated (bsm-51463M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human MDA-MB-231 cell lysates; Lane 5: Human MCF-7 cell lysates probed with SNAI1 Monoclonal Antibody, Unconjugated (bsm-51463M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human MDA-MB-231 cell lysates; Lane 5: Human MCF-7 cell lysates probed with SNAI1 Monoclonal Antibody, Unconjugated (bsm-51463M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA

Predicted Reactivity

  • Human
Overview
Catalog # bsm-51463M
Product Name SNAIL Monoclonal Antibody
Applications WB, ELISA
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human SNAI.
Clonality Monoclonal
Clone # S1F6
Concentration 0.5ug/ul
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6615
Swiss Prot O95863
Subcellular location Cytoplasm, Nucleus
Synonyms dJ710H13.1; Protein sna; Protein snail homolog; SLUGH2; SNA; Sna protein; SNAH; SNAI1; SNAI 1; Snail homolog 1 (Drosophila); Zinc finger protein SNAI1; SNAI1_HUMAN; Protein snail homolog 1; dJ710H13.1; SNAIL1; Protein sna.
Background Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.
Application Dilution
WB =1:500，ELISA=1:5000-10000
ELISA 1:500-1000

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