ALDH6A1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: MCF-7 cell lysates; Lane 2: T47D cell lysate probed with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: MCF-7 cell lysates; Lane 2: T47D cell lysate probed with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51469M
Product Name	ALDH6A1 Monoclonal Antibody
Applications	WB, IHC-P, IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human ALDH6A1.
Clonality	Monoclonal
Clone #	AL06
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4329
Swiss Prot	Q02252
Subcellular location	Nucleus
Synonyms	Aldehyde dehydrogenase 6 family member A1; Aldehyde dehydrogenase family 6 member A1; Aldehyde dehydrogenase family 6, subfamily A1; Malonate semialdehyde dehydrogenase; Malonate-semialdehyde dehydrogenase [acylating]; Methylmalonate semialdehyde dehydrogenase [acylating] mitochondrial; Methylmalonate-semialdehyde dehydrogenase [acylating]; MGC37325; MGC40271; Mitochondrial acylating methylmalonate semialdehyde dehydrogenase; mitochondrial; MMSA_HUMAN; MMSADHA; MMSDH;
Background	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Application Dilution
WB	=1:500-1000ÔºåIHC-P=1:25ÔºåIF=1:25Ôºå
IHC-P	1:200-400
IF

Applications

Predicted Reactivity

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