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RPS6KB2 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Jurkat cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates probed with RPS6KB2 Monoclonal Antibody, Unconjugated (bsm-51475M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IHC-P
  • IF

Predicted Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-51475M
Product Name RPS6KB2 Monoclonal Antibody
Applications WB, IHC-P, IF
Predicted Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human RPS6KB2 (His-tagged).
Clonality Monoclonal
Clone # G8F7
Concentration 0.5ug/ul
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6199
Swiss Prot Q9UBS0
Subcellular location Nucleus
Synonyms 70 kDa ribosomal protein S6 kinase 2; p70 beta; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6KB; p70 S6Kbeta; p70(S6K) beta; p70-beta; p70-S6KB; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; RPS6KB2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14 beta; SRK; STK14B; KS6B2_HUMAN.
Background Phosphorylates specifically ribosomal protein S6.
Application Dilution
WB =1:500-2000，IHC-P=1:200-500，IF=1:10-50，
IHC-P 1:200-400
IF

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