ZEB1 Monoclonal Antibody

$Lane 1: A549 cell lysates probed with ZEB1 Monoclonal Antibody, Unconjugated (bsm-51479M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: A549 cell lysates probed with ZEB1 Monoclonal Antibody, Unconjugated (bsm-51479M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
Flow-Cyt

Predicted Reactivity

Human

Overview
Catalog #	bsm-51479m
Product Name	ZEB1 Monoclonal Antibody
Applications	WB, Flow-Cyt
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ZEB1
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6935
Swiss Prot	P37275
Subcellular location	Nucleus
Synonyms	AREB 6; AREB6; BZP; Delta crystallin enhancer binding factor 1; DELTA EF1; FECD6; MGC133261; Negative regulator of IL 2; Negative regulator of IL2; NIL 2 A; NIL 2 A zinc finger protein; NIL 2A; NIL-2-A zinc finger protein; NIL2A; Posterior polymorphous corneal dystrophy 3; PPCD3; Represses interleukin 2 expression; TCF 8; TCF-8; TCF8; Transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB 1; ZEB;ZEB1_HUMAN; ZFHEP; ZFHX 1A; ZFHX1A; Zinc finger E box binding homeobox 1; Zinc finger E-box-binding homeobox 1; Zinc finger homeodomain enhancer binding protein.
Background	Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
Application Dilution
WB	=1:500-2000
Flow-Cyt	Flow-Cyt=1:10-50