CDC25C Monoclonal Antibody

$Lane 1: 293 cell lysates; Lane 2: transiently transfected with the CDC25C gene probed with CDC25C Monoclonal Antibody, Unconjugated (bsm-51488M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293 cell lysates; Lane 2: transiently transfected with the CDC25C gene probed with CDC25C Monoclonal Antibody, Unconjugated (bsm-51488M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51488m
Product Name	CDC25C Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human CDC25C.
Clonality	Monoclonal
Clone #	C4D2
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	995
Swiss Prot	P30307
Subcellular location	Nucleus
Synonyms	CDC 25; Cdc 25C; CDC25; Cell division cycle 25 homolog C; Cell division cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; Phosphotyrosine phosphatase; MPIP3_HUMAN.
Background	Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
Application Dilution
WB	=1:500-2000