CALCA Monoclonal Antibody

$The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n$

The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n

Applications

Reactivity

Human

Overview
Catalog #	bsm-51512m
Product Name	CALCA Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human CALCA
Immunogen Range	51-130/141
Clonality	Monoclonal
Clone #	G8D24
Isotype	IgM
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	796
Swiss Prot	P01258
Subcellular location	Secreted
Synonyms	Katacalcin; Calcitonin; Alpha CGRP; CALC 1; CALC A; CALC1; Calcitonin related polypeptide alpha; CGRP 1; CGRP; CGRP I; CGRP-1; CGRPI; CGRP1; CT; KC; MGC126648; alpha type CGRP; calcitonin 1; calcitonin gene related peptide 1; calcitonin gene related peptide I; calcitonin isoform CT preproprotein; CALC_HUMAN.
Background	Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Katacalcin is a potent plasma calcium-lowering peptide
Application Dilution
WB	1:500-2000