RAB23 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51516m |
| Product Name | RAB23 Monoclonal Antibody |
| Applications |
WB
ELISA
FLOW-CYT
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human RAB23 (Fragment) (His-tagged). |
| Clonality | Monoclonal |
| Clone # | G6S4 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 51715 |
| Swiss Prot | Q9ULC3 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | DKFZp781H0695; HSPC137; MGC8900; Rab 23; RAB family small GTP binding protein RAB 23; RAB23, member RAS oncogene family; RAB23_HUMAN; Ras related protein Rab 23; Ras-related protein Rab-23. |
| Background | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes. |
| Application Dilution | |
| WB | =1:500-2000 |
| ELISA | ELISA=1:5000-10000 |
| Flow-Cyt | Flow-Cyt=1:25 |
Frequently asked questions
Which applications is RAB23 Monoclonal Antibody (bsm-51516M) validated for, and at what dilution?
bsm-51516M is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000); Flow-Cyt (Flow-Cyt=1:25). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAB23 Monoclonal Antibody (bsm-51516M) react with?
Tested reactivity: Human, Mouse.
How should bsm-51516M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAB23 Monoclonal Antibody (bsm-51516M)?
Recombinant human RAB23 (Fragment) (His-tagged).