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PSMA5 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4°C, DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
Overview
Catalog # bsm-51520m
Product Name PSMA5 Monoclonal Antibody
Applications WB, IHC-P, IHC-F, IF, ICC/IF
Predicted Reactivity Human, Mouse, Rat, Cow
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human PSMA5 (Fragment) (His-tagged).
Clonality Monoclonal
Clone # #REF!
Isotype IgG1
Concentration 0.5ug/ul
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5686
Swiss Prot P28066
Subcellular location Cytoplasm, Nucleus
Synonyms Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome (prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome subunit zeta; Proteasome zeta chain; PSA5_HUMAN; PSC5; ZETA; Proteasome 20S alpha 5/PSMA5.
Background The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Application Dilution
WB =1:500-1000
IHC-P IHC-P=1:400-800
IHC-F IHC-F=1:400-800
IF IF=1:10-50
ICC/IF ICC/IF=1:25

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