Glucocorticoid receptor Monoclonal Antibody

$Lane 1: Jurkat cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NIH\/3T3 cell lysates; Lane 4: Mouse Liver tissue lysates probed with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-51554M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Jurkat cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NIH\/3T3 cell lysates; Lane 4: Mouse Liver tissue lysates probed with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-51554M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51554m
Product Name	Glucocorticoid receptor Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human Glucocorticoid receptor
Immunogen Range	101-200/444
Clonality	Monoclonal
Clone #	G4H12
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	290
Swiss Prot	P04150
Subcellular location	Cytoplasm, Nucleus
Synonyms	NR3C1; glucocorticoid receptor isoform alpha; GCCR; GCR; GR; Nuclear receptor subfamily 3 group C member 1; Glucocorticoid receptor lymphocyte; GRL; Grl1; GCR_HUMAN.
Background	Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Application Dilution
WB	=1:500-1000