Creatine Kinase BB Monoclonal Antibody

$Lane 1: MDA-MB453 cell lysates; Lane 2: 293 cell lysates; Lane 3: Y79 cell lysates; Lane 4: Mouse stomach tissue lysates; Lane 5: Mouse brain tissue lysates probed with Creatine Kinase BB Monoclonal Antibody, Unconjugated (bsm-51556M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: MDA-MB453 cell lysates; Lane 2: 293 cell lysates; Lane 3: Y79 cell lysates; Lane 4: Mouse stomach tissue lysates; Lane 5: Mouse brain tissue lysates probed with Creatine Kinase BB Monoclonal Antibody, Unconjugated (bsm-51556M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Xenopus

Overview
Catalog #	bsm-51556m
Product Name	Creatine Kinase BB Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Predicted Reactivity	Xenopus
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human CKB (His-tagged).
Clonality	Monoclonal
Clone #	B12J8
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1152
Swiss Prot	P12277
Subcellular location	Cytoplasm
Synonyms	B CK; B CK; B-CK; BB-CK; BCK; Brain creatine kinase; Ckb; CKBB; CK-BB; Creatine kinase B; Creatine kinase B chain; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine Kinase BB Isoenzyme; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine phosphokinase BB; KCRB_HUMAN.
Background	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Application Dilution
WB	=1:500-1000