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NME1/NM23A Monoclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with NME1\/NM23A monoclonal antibody, unconjugated (bsm-51560M) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with NME1\/NM23A monoclonal antibody, unconjugated (bsm-51560M) at 1:800 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1\/NM23A Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1 | NM23 Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1 | NM23 Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1 | NM23 Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NME1 | NM23 Antibody, Unconjugated (bsm-51560M) at 1:150 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P

Predicted Reactivity

  • Human
Overview
Catalog # bsm-51560m
Product Name NME1/NM23A Monoclonal Antibody
Applications
WB IHC-F IF IHC-P
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human NME1 (His-tagged).
Clonality Monoclonal
Clone # T3Y2
Isotype IgG2a
Concentration Lot Dependent
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4830
Swiss Prot P15531
Subcellular location Cytoplasm, Nucleus
Synonyms nucleoside dIPhosphatase kinase A; AWD; AWD, drosophila, homolog of; GAAD; Granzyme A activated DNase; GZMA activated DNase; Metastasis inhibition factor nm23; NB; NBS; NDK A; NDP kinase A; KDKA; NDPK-A; NDPKA; NDPKB; NM23 H1; NM23 long variant, included; NM23-H1; NM23-M1; NM23B; NM23H1B; NM23H1B, included; NME1; NME1-NME2 spliced read-through transcript, included; NME2; Non metastatic cells 1 protein; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Nonmetastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process associated protein; Tumor metastatic process-associated protein; NDKA_HUMAN.
Background Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
Application Dilution
WB =1:500-1000
IHC-F IHC-F=1:100-500
IF IF=1:100-500
IHC-P IHC-P=1:100-500

Frequently asked questions

Which applications is NME1/NM23A Monoclonal Antibody (bsm-51560M) validated for, and at what dilution?

bsm-51560M is validated for WB (WB=1:500-1000); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); IHC-P (IHC-P=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-51560M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NME1/NM23A Monoclonal Antibody (bsm-51560M)?

Recombinant human NME1 (His-tagged).

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