NME1/NM23A Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: MCF-7 cell lysates probed with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: MCF-7 cell lysates probed with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51560m
Product Name	NME1/NM23A Monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human NME1 (His-tagged).
Clonality	Monoclonal
Clone #	T3Y2
Isotype	IgG2a
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4830
Swiss Prot	P15531
Subcellular location	Cytoplasm, Nucleus
Synonyms	nucleoside dIPhosphatase kinase A; AWD; AWD, drosophila, homolog of; GAAD; Granzyme A activated DNase; GZMA activated DNase; Metastasis inhibition factor nm23; NB; NBS; NDK A; NDP kinase A; KDKA; NDPK-A; NDPKA; NDPKB; NM23 H1; NM23 long variant, included; NM23-H1; NM23-M1; NM23B; NM23H1B; NM23H1B, included; NME1; NME1-NME2 spliced read-through transcript, included; NME2; Non metastatic cells 1 protein; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Nonmetastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process associated protein; Tumor metastatic process-associated protein; NDKA_HUMAN.
Background	Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500