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Prohibitin Monoclonal Antibody

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-51570m
Product Name Prohibitin Monoclonal Antibody
Applications
WB IHC-F IF IHC-P
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human Prohibitin between 1-205 amino acids.
Clonality Monoclonal
Clone # E4F12
Concentration Lot Dependent
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5245
Swiss Prot P35232
Subcellular location Cytoplasm, Cell membrane
Synonyms Mitochondrial Marker; PHB; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN.
Background Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Application Dilution
WB =1:500-1000
IHC-F IHC-F=1:400-800
IF IF=1:100-500
IHC-P IHC-P=1:25-50

Frequently asked questions

Which applications is Prohibitin Monoclonal Antibody (bsm-51570M) validated for, and at what dilution?

bsm-51570M is validated for WB (WB=1:500-1000); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500); IHC-P (IHC-P=1:25-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-51570M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Prohibitin Monoclonal Antibody (bsm-51570M)?

Recombinant human Prohibitin between 1-205 amino acids.