Prohibitin Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: 293 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: Rat Liver tissue lysates probed with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: 293 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: Rat Liver tissue lysates probed with Prohibitin Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51570m
Product Name	Prohibitin Monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Prohibitin between 1-205 amino acids.
Clonality	Monoclonal
Clone #	E4F12
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5245
Swiss Prot	P35232
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Mitochondrial Marker; PHB; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN.
Background	Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:25-50
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500