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GATA3 Monoclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with GATA3 monoclonal antibody, unconjugated (bsm-51575M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with GATA3 monoclonal antibody, unconjugated (bsm-51575M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-GATA3 antibody (bsm-51575M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-GATA3 antibody (bsm-51575M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • Flow-Cyt
  • ICC/IF

Predicted Reactivity

  • Human
Overview
Catalog # bsm-51575m
Product Name GATA3 Monoclonal Antibody
Applications
WB FLOW-CYT ICC/IF
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human GATA3.
Clonality Monoclonal
Clone # G3G4
Concentration Lot Dependent
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2625
Swiss Prot P23771
Subcellular location Nucleus
Synonyms GATA 3; GATA-3; GATA binding factor 3; GATA binding protein 3; HDR; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; GATA3_HUMAN; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3.
Background Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Application Dilution
WB =1:500-1000
Flow-Cyt Flow-Cyt=1:25
ICC/IF ICC/IF=1:25

Frequently asked questions

Which applications is GATA3 Monoclonal Antibody (bsm-51575M) validated for, and at what dilution?

bsm-51575M is validated for WB (WB=1:500-1000); Flow-Cyt (Flow-Cyt=1:25); ICC/IF (ICC/IF=1:25). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-51575M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GATA3 Monoclonal Antibody (bsm-51575M)?

Recombinant human GATA3.

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