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PAH Monoclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with PAH monoclonal antibody, unconjugated (bsm-51585M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PAH monoclonal antibody, unconjugated (bsm-51585M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PAH Monoclonal Antibody, Unconjugated (bsm-51585M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-51585m
Product Name PAH Monoclonal Antibody
Applications
WB IHC-F IF IHC-P
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human PAH
Immunogen Range 121-300/452
Clonality Monoclonal
Clone # U8Y5
Concentration Lot Dependent
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5053
Swiss Prot P00439
Subcellular location Cytoplasm
Synonyms PH; PH4H_HUMAN; Phe 4 monooxygenase; Phe-4-monooxygenase; Phenylalanine 4 hydroxylase; Phenylalanine hydroxylase; Phenylalanine-4-hydroxylase; PKU; PKU1.
Application Dilution
WB =1:500-2000
IHC-F IHC-F=1:100-500
IF IF=1:100-500
IHC-P IHC-P=1:100-500

Frequently asked questions

Which applications is PAH Monoclonal Antibody (bsm-51585M) validated for, and at what dilution?

bsm-51585M is validated for WB (WB=1:500-2000); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); IHC-P (IHC-P=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-51585M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PAH Monoclonal Antibody (bsm-51585M)?

KLH conjugated synthetic peptide derived from human PAH Immunogen range: 121-300/452.

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