RPS6 Monoclonal Antibody

$MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235\/Ser236) monoclonal antibody, unconjugated (bsm-51591M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235\/Ser236) monoclonal antibody, unconjugated (bsm-51591M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51591m
Product Name	RPS6 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human RPS6.
Clonality	Monoclonal
Clone #	J88A1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6194
Swiss Prot	P62753
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	40S ribosomal protein S6; Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal (1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein.
Background	May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Application Dilution
WB	1:500-2000