ULK3 Monoclonal Antibody

$Lane 1: Hela cell lysates; Lane 2: LNCaP cell lysates; Lane 3: Mouse testis tissue lysates; Lane 4: Rat testis tissue lysates probed with ULK3 Monoclonal Antibody, Unconjugated (bsm-51594M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Hela cell lysates; Lane 2: LNCaP cell lysates; Lane 3: Mouse testis tissue lysates; Lane 4: Rat testis tissue lysates probed with ULK3 Monoclonal Antibody, Unconjugated (bsm-51594M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51594m
Product Name	ULK3 Monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ULK3
Immunogen Range	401-472/472
Clonality	Monoclonal
Clone #	F9H12
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25989
Swiss Prot	Q6PHR2
Subcellular location	Nucleus
Synonyms	DKFZP434C131; FLJ90566; Serine/threonine-protein kinase ULK3; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); Unc-51-like kinase 3.
Background	Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
Application Dilution
WB	=1:500-1000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:25-100
IF	IF=1:25