TERF2IP Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TERF2IP Monoclonal Antibody, Unconjugated (bsm-51613M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TERF2IP Monoclonal Antibody, Unconjugated (bsm-51613M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: HepG2 cell lysates; Lane 2: A431 cell lysates probed with TERF2IP Monoclonal Antibody, Unconjugated (bsm-51613M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HepG2 cell lysates; Lane 2: A431 cell lysates probed with TERF2IP Monoclonal Antibody, Unconjugated (bsm-51613M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Predicted Reactivity

Human

Overview
Catalog #	bsm-51613M
Product Name	TERF2IP Monoclonal Antibody
Applications	WB, IHC-P
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human TE2IP between 1-399 amino acids.
Clonality	Monoclonal
Clone #	M7Y2
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	54386
Swiss Prot	Q9NYB0
Subcellular location	Cytoplasm, Nucleus
Synonyms	Dopamine receptor interacting protein 5; Dopamine receptor-interacting protein 5; DRIP 5; DRIP5; hRap1; MGC105533; RAP 1; RAP1 homolog; RAP1, yeast, homolog of antibody Repressor/activator protein 1 homolog antibody TE2IP_HUMAN; Telomeric Repeat Binding Factor 2 Interacting Protein; Telomeric repeat-binding factor 2-interacting protein 1; TERF2-interacting protein; TERF2-interacting telomeric protein 1; TRF2 Interacting Telomeric Protein RAP1; TRF2 interacting telomeric RAP1 protein; TRF2-interacting telomeric protein 1; TRF2-interacting telomeric protein.
Background	Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
Application Dilution
WB	=1:500-2000ÔºåIHC-P=1:25ÔºåIHC-F=1:25Ôºå
IHC-P	1:200-400

Applications

Predicted Reactivity

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