EIF2S1 Monoclonal Antibody

$Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human K562 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human K562 cell lysates probed with EIF2S1 Monoclonal Antibody, Unconjugated (bsm-51644M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51644M
Product Name	EIF2S1 Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human EIF2A.
Clonality	Monoclonal
Clone #	B5Y8
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300
Storage Condition	Store at 2-8C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1965
Swiss Prot	P05198
Subcellular location	Cytoplasm
Synonyms	EIF2A; CDA 02; CDA02; eIF 2 alpha; EIF 2; EIF 2A; EIF-2alpha; EIF2; EIF2alpha; eIF2S1; Eukaryotic Translation Initiation Factor 2 alpha; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit alpha; IF2A_HUMAN.
Background	Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Application Dilution
WB	=1:500-2000Ôºå

Applications

Predicted Reactivity

Search our catalog of hundreds of thousands of products