ME2 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ME2 Monoclonal Antibody, Unconjugated (bsm-51670M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ME2 Monoclonal Antibody, Unconjugated (bsm-51670M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bsm-51670M
Product Name	ME2 Monoclonal Antibody
Applications	WB, IHC-P
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ME2
Immunogen Range	501-584/584
Clonality	Monoclonal
Clone #	J9E1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4200
Swiss Prot	P23368
Subcellular location	Cytoplasm
Synonyms	Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD(+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD(+) dependent mitochondrial; MAOM_HUMAN; ME 2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase.
Background	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Application Dilution
WB	=1:500-4000ÔºåIHC-P=1:25Ôºå
IHC-P	1:200-400

Applications

Predicted Reactivity

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