MBD2 Monoclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bsm-51671m |
| Product Name | MBD2 Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human MBD2 between 10-228 amino acids. |
| Clonality | Monoclonal |
| Clone # | G7R2 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 8932 |
| Swiss Prot | Q9UBB5 |
| Subcellular location | Nucleus |
| Synonyms | Demethylase; DMTase; MBD 2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41. |
| Background | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| Application Dilution | |
| WB | =1:500-4000 |
| IHC-F | IHC-F=1:25 |
| IF | IF=1:25 |
| IHC-P | IHC-P=1:25 |
Frequently asked questions
Which applications is MBD2 Monoclonal Antibody (bsm-51671M) validated for, and at what dilution?
bsm-51671M is validated for WB (WB=1:500-4000); IHC-F (IHC-F=1:25); IF (IF=1:25); IHC-P (IHC-P=1:25). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-51671M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MBD2 Monoclonal Antibody (bsm-51671M)?
Recombinant human MBD2 between 10-228 amino acids.