LIN28B Monoclonal Antibody

$Lane 1: 293 cell lysates; Lane 2: K562 cell lysates; Lane 3: HepG2 cell lysates probed with LIN28B Monoclonal Antibody, Unconjugated (bsm-51681M) at 1:4000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: 293 cell lysates; Lane 2: K562 cell lysates; Lane 3: HepG2 cell lysates probed with LIN28B Monoclonal Antibody, Unconjugated (bsm-51681M) at 1:4000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51681m
Product Name	LIN28B Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human LIN28B.
Clonality	Monoclonal
Clone #	K7L3
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	389421
Swiss Prot	Q6ZN17
Subcellular location	Cytoplasm, Nucleus
Synonyms	Lin-28B; LN28B_HUMAN; CSDD 2; CSDD2; FLJ16517; Lin 28 homolog B (C. elegans); Lin 28.2; Lin-28B; Protein lin-28 homolog B.
Background	Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Application Dilution
WB	=1:500-2000