RBCK1 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51685m |
| Product Name | RBCK1 Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant human RBCK1 (UBCE7IP3). |
| Clonality | Monoclonal |
| Clone # | R5E2 |
| Concentration | Lot Dependent |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 10616 |
| Swiss Prot | Q9BYM8 |
| Subcellular location | Nucleus |
| Synonyms | C20orf18; HOIL1_HUMAN; RanBP-type and C3HC4-type zinc finger-containing protein 1; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; HOIL-1; Hepatitis B virus X-associated protein 4; RING finger protein 54; RING-type E3 ubiquitin transferase HOIL-1; Ubiquitin-conjugating enzyme 7-interacting protein 3; RNF54; UBCE7IP3; XAP3; XAP4. |
| Background | Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteoasome. Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates. LUBAC conjugates linear polyubiquitin to IKBKG at 'Lys-285' and 'Lys-309' and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types. |
| Application Dilution | |
| WB | =1:500-2000 |
Frequently asked questions
Which applications is RBCK1 Monoclonal Antibody (bsm-51685M) validated for, and at what dilution?
bsm-51685M is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RBCK1 Monoclonal Antibody (bsm-51685M) react with?
Tested reactivity: Human.
How should bsm-51685M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RBCK1 Monoclonal Antibody (bsm-51685M)?
Recombinant human RBCK1 (UBCE7IP3).