PU.1/Spi1 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PU.1\/Spi1 monoclonal antibody, unconjugated (bsm-51687M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PU.1\/Spi1 monoclonal antibody, unconjugated (bsm-51687M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51687m
Product Name	PU.1/Spi1 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human SPI1.
Clonality	Monoclonal
Clone #	K8J9
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6688
Swiss Prot	P17947
Subcellular location	Nucleus
Synonyms	31 kDa Transforming Protein; 31 kDa-transforming protein; Hematopoietic transcription factor PU.1; OF; SFPI1; SPI 1 proto oncogene; SPI A; SPI1_HUMAN; Spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1.
Background	Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.
Application Dilution
WB	=1:500-2000