BRCA2 Monoclonal Antibody

$Lane 1: Recombinant protein lysates probed with BRCA2 Monoclonal Antibody, Unconjugated (bsm-51693M) at 1:2000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Recombinant protein lysates probed with BRCA2 Monoclonal Antibody, Unconjugated (bsm-51693M) at 1:2000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Recombinant protein

Predicted Reactivity

Human

Overview
Catalog #	bsm-51693m
Product Name	BRCA2 Monoclonal Antibody
Applications	WB
Reactivity	Recombinant protein
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human BRCA2_HUMAN.
Clonality	Monoclonal
Clone #	F2H5
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	675
Swiss Prot	P51587
Subcellular location	Nucleus
Synonyms	BRCA 2; BRCA1/BRCA2 containing complex subunit 2; BRCC 2; BRCC2; Breast and ovarian cancer susceptibility gene early onset; Breast cancer 2 early onset; Breast Cancer 2 tumor suppressor; Breast cancer susceptibility protein BRCA2; Breast cancer type 2 susceptibility protein; FACD; FAD 1; FAD; FAD1; FANCB; FANCD 1; FANCD; FANCD1; Fanconi anemia complementation group D1; Fanconi anemia group D1 protein; OTTHUMP00000018803; OTTHUMP00000042401; BRCA2_HUMAN.
Background	Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. In concert with NPM1, regulates centrosome duplication.
Application Dilution
WB	=1:500-2000