PURB Monoclonal Antibody

$Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: NCI-H460 cell lysates; Lane 4: HepG2 cell lysates; Lane 5: Mcf-7 cell lysates; Lane 6: NIH\/3T3 cell lysates probed with PURB Monoclonal Antibody, Unconjugated (bsm-51738M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: NCI-H460 cell lysates; Lane 4: HepG2 cell lysates; Lane 5: Mcf-7 cell lysates; Lane 6: NIH\/3T3 cell lysates probed with PURB Monoclonal Antibody, Unconjugated (bsm-51738M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51738m
Product Name	PURB Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human PURB.
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5814
Swiss Prot	Q96QR8
Subcellular location	Nucleus
Synonyms	Pur beta; PURB_HUMAN; PURBETA; Purine rich element binding protein B; Purine-rich element-binding protein B; transcriptional activator protein Pur beta; Transcriptional activator protein Pur-beta.
Background	Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs.
Application Dilution
WB	=1:1000-4000