Ighg | Mouse IgG3 (Isotype Control) Monoclonal Antibody, Cy5 Conjugated
Applications
| Overview | |
| Catalog # | bsm-51774m-cy5-100ul |
| Product Name | Ighg | Mouse IgG3 (Isotype Control) Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Specifications | |
| Conjugation | Cy5 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG3 |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Application Dilution | |
| WB | 1:1000-10000 |
| IF | 1:200-1000 |
| Flow-Cyt | 1:100-200 |
Frequently asked questions
Which applications is Ighg | Mouse IgG3 (Isotype Control) Monoclonal Antibody, Cy5 Conjugated (bsm-51774M-Cy5) validated for, and at what dilution?
bsm-51774M-Cy5 is validated for WB (1:1000-10000); IF (1:200-1000); Flow-Cyt (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-51774M-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-51774M-Cy5)?
Cy5 on bsm-51774M-Cy5: excitation / emission 625,650nm/670nm.