HP1 gamma Recombinant Antibody

$4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HP1 gamma) monoclonal Antibody, unconjugated (bsm-51782R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HP1 gamma) monoclonal Antibody, unconjugated (bsm-51782R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$25 ug total protein per lane of various lysates (see on figure) probed with CBX3 | HP1 gamma monoclonal antibody, unconjugated (bsm-51782R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CBX3 | HP1 gamma monoclonal antibody, unconjugated (bsm-51782R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-51782r-100ul
Product Name	HP1 gamma Recombinant Antibody
Applications	IF ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human HP1 gamma Protein
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	11335
Swiss Prot	Q13185
Subcellular location	Nucleus
Synonyms	HECH; HP1-GAMMA; HP1Hs-gamma; HP1gamma; HP1g; M32; CBX3_HUMAN; CBX3; Heterochromatin protein 1 homolog gamma (HP1 gamma); Modifier 2 protein; CBX3_MOUSE; chromobox 3; chromobox homolog 3 (Drosophila HP1 gamma); chromobox homolog 3; HP1 gamma homolog (Drosophila)
Application Dilution
IF	1:100-500
ICC/IF	1:50-200