Oct 4 (1F4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52001m-100uL |
| Product Name | Oct 4 (1F4) Monoclonal Antibody |
| Applications |
WB
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Synthetic peptide derived from human Oct 4, around 1-50aa. |
| Immunogen Range | 1-100/360 |
| Clonality | Monoclonal |
| Clone # | 1F4 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 5460 |
| Swiss Prot | Q01860 |
| Synonyms | MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer-binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding transcription factor 3; Octamer-4; POU domain transcription factor OCT4; OTF3; OTF4; OTF 3; OTF 4; OTF-3; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU domain class 5 transcription factor 1; PO5F1_HUMAN; POU type homeodomain containing DNA binding protein ; POU5F1. |
| Background | Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. |
| Application Dilution | |
| WB | 1:300-5000 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is Oct 4 (1F4) Monoclonal Antibody (bsm-52001M) validated for, and at what dilution?
bsm-52001M is validated for WB (1:300-5000); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Oct 4 (1F4) Monoclonal Antibody (bsm-52001M) react with?
Tested reactivity: Human.
How should bsm-52001M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Oct 4 (1F4) Monoclonal Antibody (bsm-52001M)?
Synthetic peptide derived from human Oct 4, around 1-50aa. Immunogen range: 1-100/360.