14-3-3 epsilon Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52003r-bf647 |
| Product Name | 14-3-3 epsilon Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | Recombinant human 14-3-3 epsilon protein, full length. |
| Clonality | Monoclonal |
| Clone # | 2E2 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7531 |
| Swiss Prot | P62258 |
| Synonyms | 14-3-3 protein epsilon; YWHAE; 14-3-3E |
| Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is 14-3-3 epsilon Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52003R-BF647) validated for, and at what dilution?
bsm-52003R-BF647 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 14-3-3 epsilon Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52003R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52003R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 14-3-3 epsilon Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-52003R-BF647)?
Recombinant human 14-3-3 epsilon protein, full length.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-52003R-BF647)?
AbBy Fluor-647 on bsm-52003R-BF647: excitation / emission 650nm/665nm.