14-3-3 sigma (2E12) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 sigma (2E12) Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 sigma (2E12) Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 sigma (2E12) Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 sigma (2E12) Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

IHC-P

Reactivity

Human

Overview
Catalog #	bsm-52005R
Product Name	14-3-3 sigma (2E12) Monoclonal Antibody
Applications	IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human 14-3-3 sigma protein, full length.
Clonality	Monoclonal
Clone #	2E+12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months..
Target
Gene ID	2810
Swiss Prot	P31947
Synonyms	14-3-3 protein sigma; SFN; Epithelial cell marker protein 1; Stratifin; HME1
Background	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.1 Publication p53-regulated inhibitor of G2/M progression.
Application Dilution
IHC-P	1:200-400

Applications

Reactivity

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