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14-3-3 Theta (5G1) Monoclonal Antibody

Lane 1: Hela; Lane 2: MCF-7 cell lysates; probed with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Hela; Lane 2: MCF-7 cell lysates; probed with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A431 cells were stained with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
A431 cells were stained with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52006R
Product Name 14-3-3 Theta (5G1) Monoclonal Antibody
Applications WB, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human 14-3-3 Theta protein, full length.
Clonality Monoclonal
Clone # 5G1
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 10971
Swiss Prot P27348
Synonyms 14-3-3 protein theta; YWHAQ; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1
Background Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Application Dilution
WB
IF(ICC) 1:50-200

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