ALAS1 Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52012r-cy55-100ul |
| Product Name | ALAS1 Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | recombinant human Alas1 C-terminal 200aa |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 211 |
| Swiss Prot | P13196 |
| Subcellular location | Cytoplasm |
| Synonyms | ALAS; ALAS-H; ALAS3; ALASH; MIG4; ALAS-N; Alas-1; ALA-S; HEM1_HUMAN; ALAS1; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1; 2.3.1.37; HEM1_MOUSE; HEM1_RAT; 5'-aminolevulinate synthase 1; aminolevulinate, delta-, synthase 1 |
| Background | This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015] |
| Application Dilution | |
| WB | 1:500-2000 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is ALAS1 Recombinant Antibody, Cy5.5 Conjugated (BSM-52012R-CY5-5) validated for, and at what dilution?
BSM-52012R-CY5-5 is validated for WB (1:500-2000); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ALAS1 Recombinant Antibody, Cy5.5 Conjugated (BSM-52012R-CY5-5) react with?
Tested reactivity: Human.
How should BSM-52012R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALAS1 Recombinant Antibody, Cy5.5 Conjugated (BSM-52012R-CY5-5)?
recombinant human Alas1 C-terminal 200aa
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-52012R-CY5-5)?
Cy5.5 on BSM-52012R-CY5-5: excitation / emission 675nm/694nm.