Aurora B Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52019r-bf680 |
| Product Name | Aurora B Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | Recombinant human Aurora B protein, around N-terminal 150aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9212 |
| Swiss Prot | Q96GD4 |
| Synonyms | Aurora B phospho Y12; Aurora B phospho T12; AIK2; AIM1; ARK2; AurB; AURKB; Aurora 1; Aurora and Ipl1 like midbody associated protein 1; Aurora kinase B; Aurora related kinase 2; Aurora-B; Aurora/IPL1 related kinase 2; IPL1; Serine/theronine kinase 12; Serine/threonine protein kinase 12; STK1; STK12; STK5. |
| Background | Mahogany (MG), originally identified as a protein involved in pigmentation, acts in conjunction with melanocortin receptors to suppress diet-induced obesity. Mahogany contains a single transmembrane domain, and it is expressed in a broad range of tissues, including the hypothalamus and pigment cells. Mutations within the mahogany gene were shown to rescue agouti-lethal-yellow mutant mice from obesity. The extracellular domain of mouse mahogany is the ortholog of the human protein attractin. Attractin (also designated DPPT-L) is a human serum glycoprotein and is a member of the CUB family of cell adhesion and guidance proteins. Attractin is expressed on activated T cells and is released from the cells 48 to 72 hours after activation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Aurora B Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52019R-BF680) validated for, and at what dilution?
bsm-52019R-BF680 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Aurora B Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52019R-BF680) react with?
Tested reactivity: Human.
How should bsm-52019R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Aurora B Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-52019R-BF680)?
Recombinant human Aurora B protein, around N-terminal 150aa.
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-52019R-BF680)?
AbBy Fluor-680 on bsm-52019R-BF680: excitation / emission 679nm/702nm.