Bcl-XL (12C5) Monoclonal Antibody

$HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Bcl-XL (12C5) Monoclonal Antibody (bsm-52024R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human A549 cell lysates probed with Bcl-XL Monoclonal Antibody, Unconjugated (bsm-5202M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52024R
Product Name	Bcl-XL (12C5) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Bcl-XL protein, around 1-100aa.
Clonality	Monoclonal
Clone #	12C5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	598
Swiss Prot	Q07817
Synonyms	BCL2L1 phospho T47; BCL2L1 phospho Thr47; p-BCL2L1 phospho T47; p-BCL2L1 Thr47; p-BCL2L1Thr115; Apoptosis regulator Bcl X; BclX; Bcl-X; Bcl 2 like 1; Bcl 2 like 1 protein; Bcl xL; BCL X/L; BCL XL/S; Bcl xS; Bcl2 like1; BCL2-related gene; BCL2-related protein, long isoform, included; BCLXL, included; BCL2-related protein, short isoform, included; BCLXS, included; BCL2L; BCL2L1; Bclx; DKFZp781P2092; MGC113803; MGC99998; B2CL1_HUMAN.
Background	The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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