Cyclin E1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52048r-rbitc |
| Product Name | Cyclin E1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Synthetic peptide derive from human cyclin e1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 898 |
| Swiss Prot | P24864 |
| Synonyms | Cyclin E1 phospho T77; p-Cyclin E1 phospho T77; Cyclin E1 Thr77; CCNE 1; CCNE;CCNE1;Cyclin E1 antibody Cyclin Es;Cyclin Et;CyclinE; G1/S specic cyclin E. |
| Background | Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Cyclin E1 Recombinant Antibody, RBITC Conjugated (bsm-52048R-RBITC) validated for, and at what dilution?
bsm-52048R-RBITC is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cyclin E1 Recombinant Antibody, RBITC Conjugated (bsm-52048R-RBITC) react with?
Tested reactivity: Human.
How should bsm-52048R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cyclin E1 Recombinant Antibody, RBITC Conjugated (bsm-52048R-RBITC)?
Synthetic peptide derive from human cyclin e1
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-52048R-RBITC)?
RBITC on bsm-52048R-RBITC: excitation / emission 570nm/595nm.