Cytochrome C Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52050r-bf488 |
| Product Name | Cytochrome C Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | Recombinant human Cytochrome C protein, full length. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54205 |
| Swiss Prot | P99999 |
| Synonyms | CYC; HCS; THC4; Cytochrome c; CYCS |
| Background | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Cytochrome C Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52050R-BF488) validated for, and at what dilution?
bsm-52050R-BF488 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytochrome C Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52050R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52050R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytochrome C Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-52050R-BF488)?
Recombinant human Cytochrome C protein, full length.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-52050R-BF488)?
AbBy Fluor-488 on bsm-52050R-BF488: excitation / emission 499nm/519nm.