Cytokeratin 1 (1C2) Monoclonal Antibody

Lane 1: 293T Cell lysates; Lane 2: HeLa cell lysates; probed with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 1 (1C2) Monoclonal Antibody (bsm-52051R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52051R
Product Name	Cytokeratin 1 (1C2) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cytokeratin 1 protein, around C-terminal 100aa.
Clonality	Monoclonal
Clone #	1C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3848
Swiss Prot	P04264
Synonyms	K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK; Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; Type-II keratin Kb1; KRT1; KRTA
Background	May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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