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Cytokeratin 17 Recombinant Antibody, Cy5 Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52057r-cy5-100uL
Product Name Cytokeratin 17 Recombinant Antibody, Cy5 Conjugated
Applications
WB IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5
Host Rabbit
Source Recombinant human Cytokeratin 17 protein, around N-terminal 100aa.
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3872
Swiss Prot Q04695
Synonyms 39.1; CK 17; Cytokeratin17; K17; Keratin 17; Keratin type I cytoskeletal 17; Keratin17; KRT 17; KRT17; KRT17 protein; PC; PC2; PCHC1; K1C17_HUMAN.
Background The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008].
Application Dilution
WB 1:300-5000
IF
Flow-Cyt 1ug/Test

Frequently asked questions

Which applications is Cytokeratin 17 Recombinant Antibody, Cy5 Conjugated (bsm-52057R-Cy5) validated for, and at what dilution?

bsm-52057R-Cy5 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cytokeratin 17 Recombinant Antibody, Cy5 Conjugated (bsm-52057R-Cy5) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-52057R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cytokeratin 17 Recombinant Antibody, Cy5 Conjugated (bsm-52057R-Cy5)?

Recombinant human Cytokeratin 17 protein, around N-terminal 100aa.

What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52057R-Cy5)?

Cy5 on bsm-52057R-Cy5: excitation / emission 625,650nm/670nm.