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Cytokeratin 4 (2F9) Monoclonal Antibody

293T cell lysates probed with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.
293T cell lysates probed with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
HeLa cells were stained with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF-7 cells were stained with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF-7 cells were stained with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

  • WB
  • IHC-P
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52062R
Product Name Cytokeratin 4 (2F9) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Cytokeratin 4 protein, around 350-480aa.
Clonality Monoclonal
Clone # 2F9
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID 3851
Swiss Prot P19013
Synonyms Keratin, type II cytoskeletal 4; KRT4
Background Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
Application Dilution
WB 1:300-1000
IHC-P 1:200-400
IF(ICC) 1:50-200

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