Cytokeratin 4 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-52062r-rbitc |
| Product Name | Cytokeratin 4 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Recombinant human Cytokeratin 4 protein, around 350-480aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3851 |
| Swiss Prot | P19013 |
| Synonyms | Keratin, type II cytoskeletal 4; KRT4 |
| Background | Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Cytokeratin 4 Recombinant Antibody, RBITC Conjugated (bsm-52062R-RBITC) validated for, and at what dilution?
bsm-52062R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytokeratin 4 Recombinant Antibody, RBITC Conjugated (bsm-52062R-RBITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-52062R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytokeratin 4 Recombinant Antibody, RBITC Conjugated (bsm-52062R-RBITC)?
Recombinant human Cytokeratin 4 protein, around 350-480aa.
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-52062R-RBITC)?
RBITC on bsm-52062R-RBITC: excitation / emission 570nm/595nm.