Cytokeratin 5 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52063r-cy5-100uL |
| Product Name | Cytokeratin 5 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | Recombinant human Cytokeratin 5 protein, around 400-500aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3852 |
| Swiss Prot | P13647 |
| Synonyms | Keratin, type II cytoskeletal 5; KRT5 |
| Background | The protein encoded is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Cytokeratin 5 Recombinant Antibody, Cy5 Conjugated (bsm-52063R-Cy5) validated for, and at what dilution?
bsm-52063R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytokeratin 5 Recombinant Antibody, Cy5 Conjugated (bsm-52063R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52063R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytokeratin 5 Recombinant Antibody, Cy5 Conjugated (bsm-52063R-Cy5)?
Recombinant human Cytokeratin 5 protein, around 400-500aa.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52063R-Cy5)?
Cy5 on bsm-52063R-Cy5: excitation / emission 625,650nm/670nm.