Cytokeratin 7 (6E6) Monoclonal Antibody

$Lane 1: A549 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: SK-BR-3 Cell lysates; Lane 4: SW1990 cell lysates; probed with Cytokeratin 7 (6E6) Monoclonal Antibody (bsm-52065R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.$

Lane 1: A549 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: SK-BR-3 Cell lysates; Lane 4: SW1990 cell lysates; probed with Cytokeratin 7 (6E6) Monoclonal Antibody (bsm-52065R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.

$A549 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 7 (6E6) Monoclonal Antibody (bsm-52065R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

A549 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 7 (6E6) Monoclonal Antibody (bsm-52065R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52065R
Product Name	Cytokeratin 7 (6E6) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cytokeratin 7 protein, around C-terminal 150aa.
Clonality	Monoclonal
Clone #	6000000
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months..
Target
Gene ID	3855
Swiss Prot	P08729
Synonyms	K7; CK7; SCL; K2C7; Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; Sarcolectin; Type-II keratin Kb7; KRT7
Background	Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Application Dilution
WB	1:300-1000
IF(ICC)	1:50-200

Applications

Reactivity

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