ERK2 Recombinant Antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52068r-pe-cy7 |
| Product Name | ERK2 Recombinant Antibody, PE-Cy7 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | Recombinant human ERK2 protein, around C-terminal 150aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55945595 |
| Swiss Prot | P28482 |
| Synonyms | ERK; p38; p4; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; Extracellular signal-regulated kinase 2; MAP kinase isoform p42; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1 |
| Background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is ERK2 Recombinant Antibody, PE-Cy7 Conjugated (bsm-52068R-PE-Cy7) validated for, and at what dilution?
bsm-52068R-PE-Cy7 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ERK2 Recombinant Antibody, PE-Cy7 Conjugated (bsm-52068R-PE-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-52068R-PE-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ERK2 Recombinant Antibody, PE-Cy7 Conjugated (bsm-52068R-PE-Cy7)?
Recombinant human ERK2 protein, around C-terminal 150aa.
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-52068R-PE-Cy7)?
PE-Cy7 on bsm-52068R-PE-Cy7: excitation / emission 488nm/780nm.