HDAC2 Recombinant Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52082r-percp-Cy5.5-100uL |
| Product Name | HDAC2 Recombinant Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | Recombinant human HDAC2 protein, around C-terminal 150aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3066 |
| Swiss Prot | Q92769 |
| Synonyms | HD2; RPD3; YAF1; Histone deacetylase 2; HDAC2 |
| Background | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is HDAC2 Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-52082R-PERCP-CY5-5) validated for, and at what dilution?
BSM-52082R-PERCP-CY5-5 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HDAC2 Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-52082R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-52082R-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HDAC2 Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-52082R-PERCP-CY5-5)?
Recombinant human HDAC2 protein, around C-terminal 150aa.
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-52082R-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-52082R-PERCP-CY5-5: excitation / emission 482nm/694nm.