HDAC3 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with HDAC3 monoclonal antibody, unconjugated (bsm-52084R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with HDAC3 monoclonal antibody, unconjugated (bsm-52084R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52084r
Product Name	HDAC3 Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human HDAC3
Clonality	Recombinant
Clone #	6F11
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8841
Swiss Prot	O15379
Subcellular location	Nucleus, Cell membrane
Synonyms	HD3; KDAC3; RPD3; RPD3-2; HDAC3_HUMAN; HDAC3; Protein deacetylase HDAC3; Protein deacylase HDAC3; SMAP45; 3.5.1.98; HDAC3_MOUSE; HDAC3_RAT; histone deacetylase 3
Background	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Application Dilution
WB	1:300-5000