HDAC6 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52086r-cy5 |
| Product Name | HDAC6 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | Recombinant human HDAC6 protein, around C-terminal 150aa. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10013 |
| Swiss Prot | Q9UBN7 |
| Synonyms | HD6; JM21; CPBHM; PPP1R90; Histone deacetylase 6; HDAC6; KIAA0901 |
| Background | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is HDAC6 Recombinant Antibody, Cy5 Conjugated (bsm-52086R-Cy5) validated for, and at what dilution?
bsm-52086R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HDAC6 Recombinant Antibody, Cy5 Conjugated (bsm-52086R-Cy5) react with?
Tested reactivity: Human.
How should bsm-52086R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HDAC6 Recombinant Antibody, Cy5 Conjugated (bsm-52086R-Cy5)?
Recombinant human HDAC6 protein, around C-terminal 150aa.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-52086R-Cy5)?
Cy5 on bsm-52086R-Cy5: excitation / emission 625,650nm/670nm.