HIF-1 beta Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52092r-bf350 |
| Product Name | HIF-1 beta Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | Recombinant human HIF-1 beta protein, around 600-700aa. |
| Clonality | Monoclonal |
| Clone # | 3F5 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 405 |
| Swiss Prot | P27540 |
| Synonyms | HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; ARNT |
| Background | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is HIF-1 beta Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52092R-BF350) validated for, and at what dilution?
bsm-52092R-BF350 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIF-1 beta Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52092R-BF350) react with?
Tested reactivity: Human.
How should bsm-52092R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF-1 beta Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-52092R-BF350)?
Recombinant human HIF-1 beta protein, around 600-700aa.
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-52092R-BF350)?
AbBy Fluor-350 on bsm-52092R-BF350: excitation / emission 346nm/442nm.