Histone H1.0 (5C11) Monoclonal Antibody

$Human lung lysates probed with Histone H1.0 (5C11) Monoclonal Antibody (bsm-52095R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.$

Human lung lysates probed with Histone H1.0 (5C11) Monoclonal Antibody (bsm-52095R) at 1:1000 overnight at 4\u02daC. Followed by a conjugated secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded human pancreas tissue, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Histone H1.0 (5C11) Monoclonal Antibody (bsm-52095R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human pancreas tissue, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Histone H1.0 (5C11) Monoclonal Antibody (bsm-52095R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52095R
Product Name	Histone H1.0 (5C11) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Histone H1.0 protein, full length.
Clonality	Monoclonal
Clone #	5C11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.
Target
Gene ID	3005
Swiss Prot	P07305
Synonyms	Histone H1.0; H1F0
Background	Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Application Dilution
WB	1:300-1000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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